20 research outputs found
Evidence of Common Cadmium and Copper Uptake Routes in Zebrafish <i>Danio rerio</i>
Cadmium
and copper accumulations in gills of zebrafish were measured
during a 48 h exposure to 0.025 μM <sup>106</sup>Cd and 0.05
or 0.5 μM <sup>65</sup>Cu as a single metal or their mixtures.
The gill transcript levels of genes involved in the transport of Cu
(CTR1 and ATP7A), Na (NHE-2), Ca (ECaC), divalent metals (DMT1), and
Zn (ZIP8) were also compared between treatments at 24 and 48 h. Cd
uptake was significantly suppressed in the presence of Cu, indicating
interaction between Cu and Cd at uptake sites, but Cu uptake was unaffected
by Cd. The decrease in Cd accumulation rates in the presence of Cu
was associated with an increase in transcript abundance of ECaC at
24 h and DMT1 at 48 h and a decrease in Zip8 transcript levels, all
known as routes for Cd uptake. Fish exposed to 0.5 μM <sup>65</sup>Cu show an increase in gill ATP7a transcript abundance, suggesting
that Cu is removed from the gill and is transferred to other organs
for detoxification. A reduction in gill CTR1 transcript abundance
was observed during the Cu–Cd exposure; this may be a regulatory
mechanism to reduce Cu loading if Cu is entering the gills by other
uptake routes, such as ECaC and DMT1